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Physiological response to three environmental stressors predicts fitness and genome quality but not base substitution rate in caenorhabditis elegans

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Title	Physiological response to three environmental stressors predicts fitness and genome quality but not base substitution rate in caenorhabditis elegans
Subject	Caenorhabditis elegans--Effect of stress on; Nematodes--Physiology; Nematodes--Genetics
Creator	Andrew, Jacob R.
Publisher	Published by Eastern Washington University Libraries. 816 F St. Cheney, WA 99004-2453. http://www.ewu.edu/library.xml
Date	2012
Type	text
Format	application/pdf
Source	Original format: MS Word.doc
Language	eng
Rights	The author retains all ownership rights to the copyright to this Master's Thesis. Copying of this document in whole or in part is allowable only for scholarly purposes, and with proper attribution given to the author. The content of this thesis shall not be used for commercial purposes, or for financial gain, without the permission of the author.
Provenance	Received from the EWU Graduate Studies Office.
Description-Table Of Contents	Abstract -- Acknowledgments -- List of Tables -- List of Figures -- Introduction -- Hypotheses -- Materials and Methods -- Results -- Discussion -- Efficacy of the Stress Treatments -- Experiment 1 -- Experiment 2.
Description-Abstract	Since DNA mutations occur all the time and may have deleterious effects on fitness (survival and reproduction), organisms expend substantial metabolic resources to find and repair mutations. This metabolic expenditure is termed the cost of fidelity. If an organism is in “poor” condition and does not have enough metabolic resources, it may no longer be able to find and repair mutations (Agrawal 2002). In this thesis, we looked at whether physiological condition, measured as survival and development in the presence of an exogenous stress (‘robustness’), correlated with mutation rate (experiments 1 and 2) or fitness (experiment 2). We measured physiological robustness of nematodes exposed to three different stressors: high heat, high free radicals, and high salt. The nematodes belonged to two Rhabditid nematode species and either had unmutated genomes (ancestral control) or genomes that had accumulated mutations. In both high heat assays, we found that heat was effective at reducing nematode survival. We found that thermotolerance was higher in the HK104 strain of Caenorhabditis briggsae than the N2 strain C. elegans. Within the N2 strain, thermotolerance correlated with fitness. In experiments 1 and 2, exposure to high free radicals and high salt reduced nematode survival and slowed nematode development. The N2 strain was significantly more tolerant of free radicals and salt than the HK104 strain. Within the N2 strain, tolerance to free radicals and salt correlated with fitness. No robustness trait significantly correlated with mutation rates in experiment 1; we could not test for correlations with future mutation rates in experiment 2 because mutation rate data are not available at this time. Overall, robustness did not predict base substitution rate but was successful at predicting fitness when fitnesses were very different.
Date-Created	2012
Format-Extent	1,027,072 bytes
Relation-Requires	Adobe Acrobat Reader
Relation-Is Part Of	EWU Theses Collection
Relation-Is Format Of	PDF of MS Word.doc. Cover page was not part of the original document; it was added when converted to PDF.
Rights-Rights Holder	Andrew, Jacob R.

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Home Physiological response to three environmental stressors predicts fitness and genome quality but...

Physiological response to three environmental stressors predicts fitness and genome quality but not base substitution rate in caenorhabditis elegans

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