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An assessment of antibiotic resistant bacteria in biosolid used as garden fertilizer

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Title	An assessment of antibiotic resistant bacteria in biosolid used as garden fertilizer
Subject	Cheney Wastewater Treatment Plant--Drug resistance in microorganisms--Washington (State)--Cheney; Sewage sludge as fertilizer--Health aspects--Washington (State)--Cheney; Sewage sludge as fertilizer--Toxicology--Washington (State)--Cheney
Creator	Hartman, Gwendolyn Lenore
Publisher	Published by Eastern Washington University Libraries. 816 F St. Cheney, WA 99004-2453. http://www.ewu.edu/library.xml
Date	2012
Type	text
Format	application/pdf
Source	Original format: MS Word.docx
Language	eng
Rights	The author retains all ownership rights to the copyright to this Master’s Thesis. Copying of this document in whole or in part is allowable only for scholarly purposes, and with proper attribution given to the author. The content of this thesis shall not be used for commercial purposes, or for financial gain, without the permission of the author.
Provenance	Received from the EWU Graduate Studies Office.
Description-Table Of Contents	Abstract -- Acknowledgements -- List of figures -- List of tables -- Introduction -- Materials and methods -- Results -- Discussion -- Conclusion -- Literature cited.
Description-Abstract	"Fertilizer grade biosolid is sold to the general public by the Cheney Wastewater Treatment Plant for use as garden fertilizer and as a soil conditioner. Moist biosolid samples were obtained from the Cheney Wastewater Treatment Plant and antibiotic resistant gram negative bacteria were isolated from it. Bacteria isolated from these samples were resistant to tetracycline, ampicillin, streptomycin, kanamycin and chloramphenicol. Tetracycline resistant bacteria were isolated on MacConkey's agar supplemented with tetracycline and their resistance to ampicillin, streptomycin, chloramphenicol and kanamycin were also determined. A total of 48 isolates cultivated to determine the presence of plasmids which often contain antibiotic resistance genes. These isolates were identified using the Vitek2 Identification System (BioMerieux, Durham N.C., U.S.A.). Sixty-seven percent of isolates were discovered to have plasmids and 54% had two or more plasmids. Plasmid size ranged from 2 to lOKb"--Document.
Date-Created	2012
Format-Extent	1,255,424 bytes
Relation-Requires	Adobe Acrobat Reader
Relation-Is Part Of	EWU Theses Collection
Relation-Is Format Of	PDF of MS Word.docx. [2] pages before title page were not part of the original document; they were added when converted to PDF. Original signatures on page ii were redacted.
Rights-Rights Holder	Hartman, Gwendolyn Lenore

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An assessment of antibiotic resistant bacteria in biosolid used as garden fertilizer

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